Fig. 5

CBP silencing downregulates CD133, cell proliferative genes and modulates PTEN-AKT pathways in TICs. a mRNA expression of CBP, CD133 and CyclinD1 in CBP silenced TICs. Results represent mean ±S.E from three independent experiments. Data was normalized to scrambled controls (SC) and represented as relative expression fold. (n = 3 #p < 0.01, *p < 0.05). b Western blot analysis to show changes in protein phosphorylation in CBP silenced cells. c Densitometry quantification of protein band intensity by Image J Software, NIH. Results represent mean ±S.E from three independent experiments. n = 3, *p < 0.05