Fig. 2

CBP-beta-catenin signaling regulates CD133 expression, cell survival proliferative genes and anchorage independent growth in HepG2 cells. a CD133 mRNA expression levels in human hepatoblastoma cell line HepG2 and cholangiocarcinoma cell line MzCha-1. Expression level was normalized to MzCha-1 cell line and fold expression was calculated, (n = 3 #p < 0.005). b Effect of time course ICG001 treatment on CD133 mRNA expression (n = 3 *p < 0.001). c Western blot to show the effect of ICG001 on CD133 protein levels. Protein band intensity was calculated by densitometry quantification by Image J Software, NIH. Results represent mean ±S.E from three independent experiments (n = 3, # p < 0.01). d FACS analysis to show the effect of ICG001 on CD133 positive cells in HepG2 cells. Data represented as % of total live cells in each group. Live cells were gated with DAPI staining. Results represent mean ±S.E from three independent experiments (n = 3, # p < 0.001). e Effect of ICG001 on CYCLIND1 and SURVIVIN expression levels in HepG2 cells. Results represent mean ±S.E from three independent experiments #p < 0.001, *p < 0.05. f Effect of ICG001 on anchorage independent growth in HepG2 cells. After 14 days of culture on soft agar with and without ICG001, colonies were stained with 0.005% crystal violet and images were captured. For each experiment 4–5 images were captured from six different experiments and colonies were counted manually and average number of colonies were shown in the graph, n = 6, # p < 0.005