Fig. 1

CBP-beta-catenin signaling regulates CD133 expression and anchorage independent growth in murine TICs: a Expression levels of CD133 mRNA in CD133 positive clonally expanded TICs from Mat1a−/− murine liver and liver cancer cell line developed from Mat1a−/− mouse liver tumors. Data represents mean ±S.E from three independent experiments (n = 3, #p < 0.0001). b Effect of time course ICG001 treatment on CD133 expression in TICs. Results represent mean ±S.E from four independent experiments (n = 4, *p < 0.005, # p < 0.0005) c Western blot to show the effect of ICG001 on CD133 protein levels. Protein band intensity was calculated by densitometry quantification by Image J Software, NIH. Data is represented as fold difference compared to DMSO treated control cells. Data represents mean ±S.E from three independent experiments (n = 3, #p < 0.005). d FACS analysis to show the effect of ICG001 on CD133 positive cells in TICs. Data represented as % of total live cells in each group. Live cells were gated with DAPI staining. Results represent mean ±S.E from three independent experiments (n = 3, # p < 0.001). e Effect of ICG001 on anchorage independent growth in TICs. After 14 days of culture on soft agar with and without ICG001, colonies were stained with 0.005% crystal violet and images were captured. For each experiment 4–5 images were captured from six different experiments and colonies were counted manually and average number of colonies were shown in the graph. n = 6, # p <  0.005