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Fig. 9 | Cell Communication and Signaling

Fig. 9

From: LncRNA HOTAIR promotes cell migration and invasion by regulating MKL1 via inhibition miR206 expression in HeLa cells

Fig. 9

MKL1 activated HOTAIR transcription by binding the CArG box in promoter region. a Diagram of the CArG box in HOTAIR promoter region. b Cells were transfected with luciferase vectors containing the promoter region of HOTAIR, along with MKL1 expression vector. Luciferase activity was measured at 48 h after transfection. HOTAIR promoter activity was decreased after transfecting with plasmids, which mutated or deleted CArG box in the promoter region of HOTAIR, compared with the control. Data are presented as means ± S.D. and results are from one representative experiment of at least three. *, P < 0.05; **, P < 0.01 (Student’s t test). c Chromatin immunoprecipitation (ChIP) assay was used to determined the interaction between HOTAIR and MKL1 in COS7 cells. Input = 5% of total lysate. IgG = immunoglobulin G

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Cell Communication and Signaling

ISSN: 1478-811X