Fig. 3

Fully differentiated M2 macrophages resist to DLL4-induced apoptosis. Macrophages were polarized into M2 macrophages with IL-4 for 48 h then cultivated for 24 h with (+rhDLL4) or without (−rhDLL4) immobilized rhDLL4. a Quantitative analysis from flow cytometry of four M2 markers (CD11b, CD163, CD200R, CD206) expressed at the surface of M2 macrophages (mean ± SD; n = 3). Results are expressed as the fold increase from 3 experiments b Representative histograms of the M2 markers expression on M2 macrophages cultivated for 72 h with IL-4 and without rhDLL4 (Black line) or 48 h plus 24 h more on immobilized rhDLL4 (Dashed line). c Apoptosis M2 macrophages. At 72 h, M2 macrophages were stained with Annexin V/Propidium Iodide (IP) and the fluorescence was measured by flow cytometry. Results are expressed as dot plots representative of 3 independent experiments