Fig. 6
From: NFAT1-regulated IL6 signalling contributes to aggressive phenotypes of glioma
IL6R is required for IL6-stimulation induced glioma cell growth and invasion. a The effect of IL6-stimulation at different concentrations on the proliferation of M1 and U87 cells, as assessed by the MTS assay. b MTS assay results showed that silencing of IL6R remarkably decreased IL6-stimulation induced proliferation of M1 cells. Scale bar = 100 μm. c The effect of IL6-stimulation at different concentrations on apoptosis of M1 and U87 cells, as examined by the TUNEL assay. d TUNEL assay demonstrated that IL6R knockdown markedly abrogated the anti-apoptotic effect of IL6 in M1 cells. Scale bar = 50 μm. e Knockdown of IL6R reversed the IL6-induced invasive potential of M1 cells, as assessed by the matrigel assay. Scale bar = 50 μm. f Wound healing assay demonstrated that silencing of IL6R significantly inhibited IL6-induced migration of M1 cells. g IL6R knockdown significantly decreased the colony formation ability of M1 cells treated with IL6 treatment. Scale bar = 50 μm. h In M1 GSCs, IL6-stimulation increased neurosphere size and number, which was also mainly dependent on the expression of IL6R. Scale bar = 50 μm. *P < 0.05 and **P < 0.01