Fig. 3

Knockdown of ATIC expression suppresses migration of HCC cells. a, HepG2 cells were transfected with indicated shRNA for 24 h followed by real-time-PCR analysis of ATIC transcription. N = 3, **, p < 0.005, ***, p < 0.001, compared to shScr and mock group. b, HepG2 cells were transfected with indicated shRNA for 72 h followed by western blot analysis of ATIC expression. The result of densitometric analysis was plotted after normalization to GAPDH. N = 3, **, p < 0.005, ***, p < 0.001, compared to shScr and mock group. c, HepG2 cells were transfected with shRNA1 for indicated times followed by western blot analysis of ATIC expression. N = 3, ***, p < 0.001, compared to shScr and mock group. d, Representative images of wound healing assay for HCC cells transfected with indicated shRNAs. The gap width at multiple constant points was measured and recorded at 12 h. Bar, 100 μm. e, Relative migration distance of cells was plotted. Migration distance in shScr group was set as 1. N = 4, **, p < 0.005. f, Representative images of transwell analyses to evaluate the migration ability of HCC cells transfected with indicated shRNAs. g, Relative migrated cell numbers were plotted after normalized to total number. N = 4, ***, p < 0.001. h, Representative images of transwell analyses to evaluate the migration ability of LO2 cells transfected with indicated shRNAs. i, Relative migrated cell numbers were plotted after normalized to total number. N = 4, NS, no significant