Fig. 7
From: Sirt1 interaction with active Smad2 modulates transforming growth factor-β regulated transcription
Sirt1 specifically repress TGFβ dependent transcriptional activity of DE-Luc reporter gene. a and b. Hep3B (a) and NIH3T3 (b) cells were transfected with the indicated plasmids. After 48 h cells were treated with TGFβ for 6 h and measured for luciferase activity. c Hep3B cells were additionally transfected with Sirt1 siRNA plasmids. After 48 h cells were treated with TGFβ for 6 h and measured for luciferase activity. “MIX”: Mixer transcription factor. “SCR”: Scramble sequence. Luciferase activity represents firefly luciferase activity normalized by Renilla activity. Asterisks denote significant differences according to ANOVA statistical analysis: *p < 0.05; ***p < 0.005 ***p < 0.001; ****p < 0.0001