Fig. 4
From: Sirt1 interaction with active Smad2 modulates transforming growth factor-β regulated transcription
Sirt1 and Smad2 co-localize in the nucleus after TGFβ treatment. a HEK293T cells were treated with SB-431542 overnight, the washed out and treated with TGFβ for the indicated times. Cells were immuno-labeled for Smad2 (green) and Sirt1 (red). Plots show Sirt1-Smad2 colocalization (pixel fluorescence intensity in each channel) for whole cells. b Plots show Sirt1-Smad2 colocalization for single HEK293T cell’s nucleus and cytoplasm separately. X-axis: Smad2-bound fluorophore intensity. Y-axis: Sirt1-bound fluorophore intensity. c HaCaT-GFP-Smad2 cells were treated with TGFβ for the indicated times, then immuno-labeled for Sirt1. Plots show Sirt1-Smad2 colocalization for single cell’s nucleus and cytoplasm. Detection areas: (1) green fluorescence; (2) red fluorescence; (3) colocalization. Color bar: absolute frequency range. All the experiments in this figure were repeated at least three times. Representative results are shown