Skip to main content
Fig. 4 | Cell Communication and Signaling

Fig. 4

From: Human hyaluronic acid synthase-1 promotes malignant transformation via epithelial-to-mesenchymal transition, micronucleation and centrosome abnormalities

Fig. 4

HAS1 expression induces micronucleation. a A2-HAS1 and HE-HAS1 has more micro-nuclei than LMA2 and Mock transfected MCF10A cells. The average number of micronucleus / 100 nuclei from the cells are presented from 6 microscopic fields (40×) with respective standard errors. b HAS1 [but not pTET (control) or HAS2 cells] induced micronucleation in transfected DLD1 cells with short-term induction. Average numbers of micronucleus/100 nuclei are presented with standard error bar. c The long-term (4 weeks) culture of Dox-induced cells (from Fig. B) continue to produce micronuclei only when HAS1s were expressed but not for cell alone (DLD1, maintained in Tet-free media for 10 weeks), pTET-control cells or HAS2 transfected cells. Average number of micronucleus / 100 nuclei are presented with standard error bar. d Sample photographs of Tet-inducible DLD1-cells induced for 100 h. The micronuclei were observed in HAS1 expressing cells only (middle panel) and indicated with arrow-heads

Back to article page

Cell Communication and Signaling

ISSN: 1478-811X