Fig. 1

Representative images of TNT formation of MSTO-211H malignant pleural mesothelioma cells in open culture (6-well standard culture plates). TNTs connecting DiI-stained MSTO cells are marked by white arrowheads. MSTO cells were first grown using standard passage medium (10% FCS RPMI-1640), then switched to a low-serum, hyperglycemic RPMI-1640 culture medium, stained with lipophilic DiI, and imaged after 48 h on a Olympus X70 fluorescent microscope at 600× magnification using phase contrast (a) and fluorescent (b) settings