Fig. 7
From: CD146, a novel target of CD44-signaling, suppresses breast tumor cell invasion
Functional validation of CD146 function in breast cancer cell invasion. Invasion of cells was demonstrated by Boyden chamber invasion assay. Note a marked induction in MCF7-B5-CD146 cell invasion capability after inhibiting CD146 expression using siRNA. Control cells were cultured in the same conditions (HA-, tet+) to induce CD146 and inhibit CD44, then it was transfected with scrambled siRNA as negative control for CD146 specific siRNA. All data have been done in triplicate and in three independent experiments. Representative images of Boyden chamber membranes represent the number of captured cells during the invasion, illustrating the difference in invaded cell numbers. The difference is considered statistically significant (student’s two-tailed t-test, **P < 0.001)