Fig. 6

Glomerular morphology. Photomicrography of representative glomeruli from normal mice (a); untreated diabetic mice (b); and TM-RH (c). Glomeruli sections (5 μm) were stained with hematoxylin and eosin and magnified × 100. TM-RH showed attenuation of glomerular hypertrophy (d), with a morphology resembling glomeruli from normal mice, reduction in mesangial thickening and a decrease in the extracellular matrix protein deposits. Scale bar = 80 μm. The data are the means ± s.d. **P < 0.01. Area distribution was calculated with ImageJ software. Normal (n = 5); Diabetic (n = 5); TM-RH (n = 3). Glomeruli analyzed per animal (n = 15 to 20)