|
Assay
|
Primer identity
|
Primer sequence (5′-3′)
|
PCR-size (bp)
|
|---|
|
Pyro-sequencing
|
IGF2-DMR0-PS-F
|
TTTTTTGTTGTATTTTGGATTTAGATTTTTT
|
186
|
|
IGF2-DMR0-PS-R
|
CTCCAAACACCCCCACCTTAA
|
|
IGF2-DMR0-Seq
|
GTGGGGAGGGGGTTTATTTTT
|
|
IGF2-H19-ICR-PS-F
|
GGGGGCTCTTGCATAGCACATGGGTA
|
242
|
|
IGF2-H19-ICR-PS-R
|
ATAAACCCTTATCCTATAAATACCCTCA
|
|
IGF2-H19-ICR-PS-Seq
|
GGTATTTTTGGAGGTTTTTTT
|
|
qMSP
|
GSTP1-MSP-U-F
|
GATGTTTGGGGTGTAGTGGTTGTT
|
91
|
|
GSTP1-MSP-U-R
|
CCACCCCAATACTAAATCACAACA
|
|
GSTP1-MSP-M-F
|
TTCGGGGTGTAGCGGTCGTC
|
97
|
|
GSTP1-MSP-M-R
|
GCCCCAATACTAAATCACGACG
|
|
RT-qPCR
|
IGF2-RTPCR-F
|
GGGCAAGTTCTTCCAATATGA
|
214
|
|
IGF2-RTPCR-R
|
TCACTTCCGATTGCTGGC
|
|
ßActin-RTPCR-F
|
CGGAGTACTTGCGCTCAGGAGGA
|
226
|
|
ßActin-RTPCR-R
|
CCTTCCTTCCTGGGAATGGAGTC
|
|
KLF4-RTPCR-F
|
ACTCGCCTTGCTGATTGTCT
|
127
|
|
KLF4-RTPCR-R
|
AATTGGCCGAGATCCTTCTT
|
|
AMACR-RTPCR-F
|
ACGACTTACAGGACAGCAGA
|
181
|
|
AMACR-RTPCR-R
|
CCTTCGTCTTCTCTGCAAAT
|
|
ChIP-qPCR
|
IGF2-DMR0-ChIP-F
|
CACCCTGGGGCCAAGGCAGT
|
100
|
|
IGF2-DMR0-ChIP-R
|
CTTGAGGGGTCATGGCACGGAAT
|
|
LOI study
|
IGF2-LOI-ApaI-F
|
CACCCCCCTCTTTCTCTTCT
|
245
|
|
IGF2-LOI-ApaI-R
|
TACTGGGTCCCTCTGACTGCT
|
|
CNV study
|
ZNF80-GCN-F
|
CTGTGACCTGCAGCTCATCCT
|
120
|
|
ZNF80-GCN-R
|
TAAGTTCTCTGACGTTGACTGATGTG
|
|
GPR15-GCN-F
|
GGTCCCTGGTGGCCTTAATT
|
101
|
|
GPR15-GCN-R
|
TTGCTGGTAATGGGCACACA
|
-
Abbreviations: qMSP quantitative methylation specific PCR, RT-qPCR reverse transcription of RNA in cDNA followed by quantitative real-time PCR, ChIP-qPCR chromatin immunoprecipitation followed by quantitative real-time PCR, IGF2-DMR0 differential methylated region in promoter P0 of the insulin-like growth factor 2 gene, IGF2/H19-ICR imprinting control region of IGF2 and H19 genes, KLF4 Kruppel-like factor 4, AMACR Alpha-methylacyl-CoA racemase, LOI loss of imprinting, CNV copy number variation, bp-base pairs
