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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: Csk-homologous kinase (Chk) is an efficient inhibitor of Src-family kinases but a poor catalyst of phosphorylation of their C-terminal regulatory tyrosine

Fig. 5

Effects of mutation of the conserved basic residues in the αD-helix and αF-αG loop region of Chk on its inhibition and binding of Hck (2PA-YEEI). a-f Sensorgrams depicting the kinetics of interactions of Chk and Chk mutants including Chk (R276A), Chk (R278A), Chk (R280A), Chk (R382A), Chk (K387A) with the immobilised Hck (2PA-YEEI). g Kinetic parameters of the interactions. h Enzymatic activities of Hck (2PA-YEEI) in the presence and absence of Chk or the Chk mutants [Chk (R276A), Chk (R382A) and Chk (K387A)] at the designated concentrations

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Cell Communication and Signaling

ISSN: 1478-811X