Fig. 3
Effects of glyceollins I and II on the expression of endogenous E2 target genes. MCF-7 cells were treated with different doses of glyceollin I (white) or II (black) with or without 10−9 M E2. The relative expression of PgR (a), GREB1 (b), TFF1 (c) and AREG (d) was assessed by real-time PCR and normalized to the expression of the housekeeping genes GAPDH and TBP. The results are expressed as the percentage of relative expression of each transcript obtained in the E2-treated cells and are represented as the mean +/− SEM of four independent experiments. *p-value <0.05, **p-value <0.01 and ***p-value <0.001 with a Mann-Whitney test followed by Bonferroni correction for comparisons of the control vs the treatments. #p-value <0.05 with a Mann-Whitney test followed by Bonferroni correction for comparisons of the E2 treatment vs the other treatments