Table 1 List of primer sequences used for genotyping, ChIP-PCR and qRT-PCR
From: Kaiso differentially regulates components of the Notch signaling pathway in intestinal cells
Experiment | Target | Primer sequence 5′-3′ |
|---|---|---|
Genotyping | fSV40-Kaiso | ATCATCAAAGCCGGGTGGGCA |
rSV40-Kaiso | TTTTCTACTCTCCATTTCATTCAAGTCCTC | |
ChIP | DLL1KBS F | AAGCTCTGCAGCTCTCTTGG |
DLL1KBS R | GGCGACTTTCGTTTTCCTC | |
JAG1KBS B F | AGCTCTTGTGGCCTCACTTC | |
JAG1KBS B R | CCTCAGGCACTACCTCCAGA | |
JAG1KBS C F | CCTGAGGGTGTAAGTGATAGGC | |
JAG1KBS C R | GAGGAAAGGGAAATGTTGGG | |
qRT-PCR | mDll-1 F | GCGACTGAGGTGTAAGATGGAA |
mDll-1 R | TCTCAGCAGCATTCATCGGG | |
mDll-4 F | GCAAACTGCAGAACCACACA | |
mDll-4 R | TGGCTTCTCACTGTGTAACCG | |
mNotch1 F | ACAGTGCAACCCCCTGTATG | |
mNotch1 R | TCTAGGCCATCCCACTCACA | |
mNotch2 F | ACAGTGTTGGCTCCCTGTTC | |
mNotch2 R | ATCGTTTACCTTGCCAGCCA | |
mHMBS F | GATGGGCAACTGTACCTGACTG | |
mHMBS R | CTGGGCTCCTCTTGGAATG | |
hDll1 F | AGAAAGTGTGCAACCCTGGC | |
hDll-1 R | CACTCTGCACTTGCATTCCCC | |
hJag1 F | CGCAAGCGATGTAGATTGAATATT | |
hJag1 R | CGCAAGCGATGTAGATTGAATATT | |
β-Actin F | CTCTTCCAGCCTTCCTTCCT | |
β-Actin R | AGCACTGTGTTGGCGTACAG |