Fig. 6
From: Metabolic shift in density-dependent stem cell differentiation
Differential protein expression of cell adhesion molecules during density-dependent differentiation identifies molecules related to cellular adhesion and formation of vascularization. Protein expression analysis of induced vascular progenitor cells (VPCs) derived from embryonic stem cells (ESCs) comparing seeding densities of 1,000 vs 10,000 cells/cm2. Flow cytometric cell scanning plots of 100,000 events of differentiating FLK1+ embryonic stem cells (a) seeded at low density of 1,000 cells/cm2 and (b) seeded at high density of 10,000 cells/cm2. The FLK1+ cells were gated in quadrant 1 (Q1) for FLK1+/CDH2+CDH5−, in quadrant 2 (Q2) for FLK1+/CDH2+CDH5+, in quadrant 3 (Q3) for FLK1+/CDH2−CDH5−, and in quadrant 4 (Q4) for FLK1+/CDH2−CDH5+. Markers were quantified by fluorescence of FLK1 allophycocyanin conjugate (APC), CDH2 fluorescein isothiocyanate (FITC) conjugate and CDH5 brilliant violet (BV) conjugate. c Fold change of FLK1+/CDH2+CDH5+ gated in Q2 with significant differential expression of cells derived from seeding at densities of 1,000 vs 10,000 cells/cm2 indicated by asterisk * with p-value below 0.05