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Fig. 9 | Cell Communication and Signaling

Fig. 9

From: Senescence-associated IL-6 and IL-8 cytokines induce a self- and cross-reinforced senescence/inflammatory milieu strengthening tumorigenic capabilities in the MCF-7 breast cancer cell line

Fig. 9

MDA-MB-231 cells exhibited incomplete/aberrant stem-like cell characteristics. a Sphere formation assay in the presence of defined medium (EGF and FGF) for the MCF-7 and MDA-MB-231 cell lines. Representative micrographs (10, 20 and 40×) show sphere formation after 9 days of induction. Scale bar, 100 μm (b). Differentiation assay of MCF-7 and MDA-MB-231 cells. Differentiation was evaluated after 10 days of induction. Osteoblasts (ALP activity), adipocytes (oil red-O) and chondrocytes (Safranin O) differentiation was determined with specific staining. Representative images are shown. Scale bar, 10 μm. c RUNX2 and APL gene expression determined at day 3, 6 and 9 of differentiation by qRT–PCR. The values were normalized to GADPH and relative to control cells. Error bars represent SEM (*p < 0.01; (**p < 0.001) (n = 2)

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Cell Communication and Signaling

ISSN: 1478-811X