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Fig. 8 | Cell Communication and Signaling

Fig. 8

From: Senescence-associated IL-6 and IL-8 cytokines induce a self- and cross-reinforced senescence/inflammatory milieu strengthening tumorigenic capabilities in the MCF-7 breast cancer cell line

Fig. 8

The treatment with IL6 and IL8 induces senescence in MCF-7 cells. a Representative images of MCF-7 cells treated with SCM during 10 days or (c) cytokines (50 ng/ml) during 5 days and stained for SA-β-GAL. Scale bar, 10 μm. b Gene expression profile of p16, p21 and p53 in MCF-7 cells stimulated with SCM or (e) cytokines, as indicated. The values were normalized to GADPH and relative to control cells (dotted lines). Error bars represent SEM. (*p < 0.05; **p < 0.01; ***p < 0.001) (n = 2). The incubation of SCM with neutralizing anti-IL6 and anti-IL8 antibodies suppresses SA-βGal activity (d) and reduce the expression of senescent markers P16, P21 and P53 (f) determined by qRT-PCR. Error bars represent SEM. (**p < 0.01; ***p < 0.001) (n = 2)

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Cell Communication and Signaling

ISSN: 1478-811X