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Fig. 6 | Cell Communication and Signaling

Fig. 6

From: Senescence-associated IL-6 and IL-8 cytokines induce a self- and cross-reinforced senescence/inflammatory milieu strengthening tumorigenic capabilities in the MCF-7 breast cancer cell line

Fig. 6

MCF-7 cells treated with IL6 and IL8 exhibit differentiation potential characteristics and express reprogramming factors. a Differentiation to mesenchymal cell lineages was induced by using specific induction media as described in Materials and Methods and evaluated after 10 days of induction. Osteoblasts (ALP activity), adipocytes (oil red-O) and chondrocytes (Safranin O) differentiation was determined with specific staining. Representative images are shown. Scale bar, 10 μm. b Gene expression levels of reprogramming-associated transcription factors in MCF-7 cells stimulated with cytokines as indicated and determined by qRT–PCR. The values were normalized to GADPH and relative to control cells (dotted lines). Error bars represent SEM (*p < 0.05) (n = 2)

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Cell Communication and Signaling

ISSN: 1478-811X