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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Senescence-associated IL-6 and IL-8 cytokines induce a self- and cross-reinforced senescence/inflammatory milieu strengthening tumorigenic capabilities in the MCF-7 breast cancer cell line

Fig. 2

The treatment with IL6 and IL8 induced morphological and phenotypic changes associated with an EMT process. a MCF-7 cells stimulated with 50 ng/ml of IL6, IL8 or IL6 + IL8 cytokines. 5 ng/ml of TGF-β was used as a positive control and MCF-7 cultured in MEM-α medium supplemented with 0,5% FBS was used as a negative control. The evaluation was done after 96 h of cytokine treatment. Representative images are shown. Arrowhead indicates cells with fibroblastoid morphology. Scale bar, 10 μm. b Representative plot of fluorescence intensity of the EMT surface markers was determined by FACS using a CD44-FITC and a CD24-PE monoclonal antibody in MCF-7 cells stimulated for 5 days with cytokines (n = 2)

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