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Table 1 Antibodies used for western blot analyses and immunohistochemistry with their sources, dilutions and manufacturers

From: The role of apoptosis repressor with a CARD domain (ARC) in the therapeutic resistance of renal cell carcinoma (RCC): the crucial role of ARC in the inhibition of extrinsic and intrinsic apoptotic signalling

Protein Source Dilution Manufacturer
Bcl-A1 Mouse, monoclonal 1:500 Santa Cruz, Germany
ARC (IH) Rabbit, poloyclonal 1:2 000 Santa Cruz, Germany
ARC (WB) Rabbit, polyclonal 1:1 000 Thermo Scietific, Germany
β-Actin Mouse, monoclonal 1:10 000 DAKO, Germany
Bad Rabbit, polyclonal 1:1 000 Cell Signalling, Germany
Bak Rabbit, polyclonal 1:1,000 Cell Signalling, Germany
Bax Rabbit, polyclonal 1: 1 000 Cell Signalling, Germany
Bcl-2 Mouse, monoclonal 1:1 000 DAKO, Germany
Bcl-w Rabbit, polyclonal 1:1 000 Santa Cruz, Germany
Bcl-xl Rabbit, polyclonal 1:1 000 Cell Signalling, Germany
Bid Rabbit, polyclonal 1:1 000 Cell Signalling, Germany
Bim Rabbit, polyclonal 1:1 000 Cell Signalling, Germany
Bok Rabbit, polyclonal 1:1 000 Cell Signalling, Germany
GAPDH Rabbit, polyclonal 1:4 000 Sigma, Germany
Mcl-1 Rabbit, polyclonal 1:1 000 Cell Signalling, Germany
Phospho-p44/42 (Thr202/Tyr204) Rabbit, polyclonal 1:1 000 Cell Signalling, Germany
PUMA Rabbit, polyclonal 1:1 000 Cell Signalling, Germany
α-Tubulin Mouse, monoclonal 1:10 000 Sigma, Germany
Secondary antibodies
 Rabbit Goat, polyclonal 1:10 000 LI-COR, Germany
 Mouse Goat, polyclonal 1:10 000 LI-COR, Germany
 Rabbit, AlexaFluor 514 Goat, polyclonal 1:1 000 Invitrogen, Germany
 mMouse, AlexaFluor 594 Goat, polyclonal 1:1 000 Invitrogen, Germany