Table 1 Antibodies used for western blot analyses and immunohistochemistry with their sources, dilutions and manufacturers
Protein | Source | Dilution | Manufacturer |
|---|---|---|---|
Bcl-A1 | Mouse, monoclonal | 1:500 | Santa Cruz, Germany |
ARC (IH) | Rabbit, poloyclonal | 1:2 000 | Santa Cruz, Germany |
ARC (WB) | Rabbit, polyclonal | 1:1 000 | Thermo Scietific, Germany |
β-Actin | Mouse, monoclonal | 1:10 000 | DAKO, Germany |
Bad | Rabbit, polyclonal | 1:1 000 | Cell Signalling, Germany |
Bak | Rabbit, polyclonal | 1:1,000 | Cell Signalling, Germany |
Bax | Rabbit, polyclonal | 1: 1 000 | Cell Signalling, Germany |
Bcl-2 | Mouse, monoclonal | 1:1 000 | DAKO, Germany |
Bcl-w | Rabbit, polyclonal | 1:1 000 | Santa Cruz, Germany |
Bcl-xl | Rabbit, polyclonal | 1:1 000 | Cell Signalling, Germany |
Bid | Rabbit, polyclonal | 1:1 000 | Cell Signalling, Germany |
Bim | Rabbit, polyclonal | 1:1 000 | Cell Signalling, Germany |
Bok | Rabbit, polyclonal | 1:1 000 | Cell Signalling, Germany |
GAPDH | Rabbit, polyclonal | 1:4 000 | Sigma, Germany |
Mcl-1 | Rabbit, polyclonal | 1:1 000 | Cell Signalling, Germany |
Phospho-p44/42 (Thr202/Tyr204) | Rabbit, polyclonal | 1:1 000 | Cell Signalling, Germany |
PUMA | Rabbit, polyclonal | 1:1 000 | Cell Signalling, Germany |
α-Tubulin | Mouse, monoclonal | 1:10 000 | Sigma, Germany |
Secondary antibodies | |||
 Rabbit | Goat, polyclonal | 1:10 000 | LI-COR, Germany |
 Mouse | Goat, polyclonal | 1:10 000 | LI-COR, Germany |
 Rabbit, AlexaFluor 514 | Goat, polyclonal | 1:1 000 | Invitrogen, Germany |
 mMouse, AlexaFluor 594 | Goat, polyclonal | 1:1 000 | Invitrogen, Germany |