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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: The role of apoptosis repressor with a CARD domain (ARC) in the therapeutic resistance of renal cell carcinoma (RCC): the crucial role of ARC in the inhibition of extrinsic and intrinsic apoptotic signalling

Fig. 3

Expression of ARC and Bcl-2 family members and ABT263-induced apoptosis in RCC cell lines. a ARC as well as anti-apoptotic Bcl-2 family members were expressed in RCC cell lines, but expression intensity differed between these cell lines. Overall, Bcl-2, Bcl-xl and Mcl-1 exhibited the strongest expression, whereas Bcl-w showed weaker expression. Bcl-2 was not expressed in clearCa-3 cells and Bcl-w was not expressed in clearCa-12 cells. BCL-A1 could not be detected (not shown). The p53 mutational status of the cell lines were published by our group elsewhere [39, 40]. b RCC cell lines expressed pro-apoptotic Bcl-2 family members of all functional groups. Sensitisers (Bid, Bim), activators (Puma, Bad, Bok) and effectors (Bax, Bak) were detectable in all RCC cell lines, also with differences in the intensity of expression, with clear expression of PUMA only in clearCa-3, clearCa-6 and clearCa-11 and Bok only in clearCa-6, claerCa-7 and clearCa-11. c All RCC cell lines revealed sensitivity towards ABT263-induced cell death (10 and 20 μM ABT263), as determined by cell count. All values are expressed as the mean ± s.d. *p < 0.05. d In 3 arbitrarily selected cell lines (clearCa-3, −6 and −12) ABT263 (10 μM) induced mitochondrial apoptosis as determined by caspase-9 and mitochondrial activation (JC-1 stain). All values are expressed as the mean ± s.d. *p < 0.05

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