Fig. 4

Induction of TCTP expression through 5-FU or oxaliplatin is inhibited by mTOR kinase inhibitors. HCT116 cells were treated for 48 h with 5-FU or oxaliplatin at the indicated concentrations. Where indicated, AZD 8055 (100 nM) or PP242 (1 μM) were added. TCTP levels were assessed by western blotting; signals were quantified and normalised against α-tubulin as a loading control. As a readout of mTOR activity, phosphorylation of the ribosomal protein S6 at Ser 240/244 was assessed using a phospho-specific antibody. a Representative western blots demonstrating the alteration of the levels of TCTP protein and of phospho-ribosomal protein S6, respectively, in relation to the drug concentration. b Graphs, showing the alteration of TCTP levels (top graphs) and relative rpS6 phosphorylation levels (bottom graphs) against the drug concentration, both in the absence (open bars) or in the presence of the mTOR kinase inhibitors AZD8055 (dark grey bars) or PP242 (light grey bars). Bars represent the average of three experiments ± SD