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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: HtrA-mediated E-cadherin cleavage is limited to DegP and DegQ homologs expressed by gram-negative pathogens

Fig. 1

E-cadherin cleavage during infection with Gram-negative pathogens. Human epithelial cells were infected with (a) H. pylori (Hp) at a MOI 100, (b) EPEC (Ep) at a MOI 5, (c) S. Typhimurium (St) at a MOI 5, (d) Y. enterocolitica (Ye) at a MOI 50 and (e) P. mirabilis (Pm) at a MOI 2. Different MOIs were chosen after careful titration of infection doses to minimize bacterial overgrowth during infection. After indicated time periods, cells were lysed and full length E-cadherin (EcadFL) was detected by Western blot analyses using an antibody against the intracellular domain. Aliquots of supernatants were analyzed for the soluble extracellular E-cadherin fragment (EcadNTF) using an antibody against the extracellular domain. β-actin served as a loading control

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Cell Communication and Signaling

ISSN: 1478-811X