Fig. 2

Effects of Tyro3, Axl and Mertk on Cell adhesion, migration and invasion. a-c Parental and EGFR/TAM CHO cells adhesion (a), migration (b) and invasion (c), in response to EGF stimulation analyzed by xCELLigence system. d Immunoblot analysis of Axl expression showing CRISPR/Cas9 efficiently disrupts Axl in 4 T1 cell clones 1.2, 1.4, 2.2 and 2.3. 4 T1 Axl knockout clone 2.2 was used in subsequent experiments. e The effect of Axl knockout on cell growth compared to 4 T1 WT as determined by assessing cell proliferation using an MTT assay for upto 96 h. Mean values ± SD are shown (n = 5). f BALB/C mice were injected in mammary fat pad with 5 × 10⁴ 4 T1 WT or 4 T1 Axl KO clone 2.2 cells, 8 mice per group. On day 7 after cancer cell implantation, tumor volume measurements began and were performed every 3 days. Mean ± SD is shown, *P < 0.05 by Student’s t-test. g Quantification of microscopic nodules in the lungs of each group and data presented as the mean ± SE. Statistical analysis was performed using Student’s t- test. h Representative lung samples showing metastatic nodules in 4 T1 WT vs 4 T1 Axl KO groups. i Total body weights were not significantly different between the 4 T1 WT and 4 T1 Axl KO groups