Fig. 4
From: Role of PKCtheta in macrophage-mediated immune response to Salmonella typhimurium infection in mice
PKCθ is involved in bacterial killing of macrophages after infection with S. typhimurium via inhibition of IL-10 deregulation. a Killing capacity of BMDMs from WT versus PKCtheta −/− mice was investigated after infection of macrophages with S. typhimurium. Macrophages were treated with IL-10 or IgG control antibodies. Significantly enhanced numbers of colony-forming units (cfu) were found in lysates of macrophages from PKCtheta −/− mice compared to WT mice. This effect could be abrogated with IL-10 blockade. Data represent the mean of at least three independent experiments ± s.e.m. *p < 0.05; b PKC inhibitor AEB071 enhanced cfu in macrophages infected with S. typhimurium highly significantly as shown in a killing experiment with BMDM from WT mice. DMSO served as control vehicle. Error bars denote the mean ± s.e.m. ***p < 0.001