Fig. 1

PKCtheta mRNA levels are transiently induced in an LPS/IFNgamma-dependent manner in both mouse and human macrophages: a BMDMs were differentiated and polarized to CAMs (LPS/IFNgamma) and AAMs (IL-4). PKCtheta gene expression was determined by qRT-PCR after different time points and was found to be selectively expressed under CAM polarization. Data shown are derived from at least three independent experiments and qRT-PCR data were normalized to Gapdh. Error bars denote the mean ± s.e.m. *p < 0.05; ** p < 0.01; Expression of unstimulated macrophages was arbitrarily set to 1. b + c In order to control proper polarization of macrophages, gene expression of prominent markers (iNOS for CAM, Fizz-1 for AAM) were validated. d CD14+ human monocytes from peripheral blood were differentiated and polarized with LPS/IFNgamma. After predetermined time periods, gene expression of PKCtheta was analysed with qRT-PCR, revealing an increase in PKCtheta mRNA levels upon stimulation in the human setting, consistent with results from mouse experiments. Data shown are relative to expression of unstimulated macrophages, which was arbitrarily set to 1. All data were normalized to Gapdh