Fig. 2
From: The NESH/Abi-3-based WAVE2 complex is functionally distinct from the Abi-1-based WAVE2 complex
Ectopically expressed NESH/Abi-3 was incorporated into the WAVE2 complex. (a) Twenty μg aliquots of lysates prepared from the control and FLAG-NESH-expressing cells were analyzed by Western blotting with the indicated antibodies. For Abi-2, data regarding 100 μg aliquots of lysates are also shown because no signal was found when 20 μg aliquots were used. (b) The lysates of the control and FLAG-NESH-expressing cells were immunoprecipitated with mouse IgG, an anti-Abi-1 (1G9), an anti-NESH (2H7), or an anti-FLAG (M2) antibody. The precipitated proteins were analyzed by Western blotting with the indicated antibodies. To confirm the NESH precipitation, a rabbit anti-human NESH antibody was also used