Table 1 Primers used for RT-PCR
From: Pasteurella multocida toxin- induced osteoclastogenesis requires mTOR activation
Gene | Forward | Reverse |
|---|---|---|
Acp5 | 5´-TTC CAG GAG ACC TTT GAG GA-3´ | 5´-TTC CAG GAG ACC TTT GAG GA-3´ |
Oscar | 5´-AGG GAA ACC TCA TCC GTT TG-3´ | 5´-GAG CCG GAA ATA AGG CAC AG-3´ |
Ctsk | 5´-AGG GAA GCA AGC ACT GGA TA −3´ | 5´-GCT GGC TGG AAT CAC ATC TT-3´ |
Atp6v0d2 | 5´-TCA GAT CTC TTC AAG GCT GTG CTG-3´ | 5´-GTG CCA AAT GAG TTC AGA GTG ATG-3´ |
Nfatc1 | 5´-GGG TCA GTG TGA CCG AGG AT-3´ | 5´-GGA AGT CAG AAG TGG GTG GA-3´ |
S29 | 5´-AGC CGA CTC GTT CCT TTC TC-3´ | 5´-CGT ATT TGC GGA TCA GAC C-3´ |
Pdcd4 | 5´-CAC TCA TAC TCT GTT CTT −3´ | 5´-`TCC ATC TCC TTC ACT TAC −3´ |