Fig. 5

TSAd affects Nck/SLP-76 interaction. Immunoblot of Nck-IP and lysates from JTAg cells with TSAd expression (siRNA Control) or JTAg cells lacking TSAd (siRNA TSAd), as in Fig. 4b. a Nck-IPs of the same cells shown in Fig. 4b were immunoblotted with the indicated antibodies. Western blots are from one representative out of four (unstimulated) or three (anti-CD3 stimulated) independent experiments. b Densitometry analysis of the relative amounts of SLP-76 levels co-immunoprecipitated with anti-Nck antibody from (a) was performed by ImageJ analysis. The amount of co-immunoprecipitated SLP-76 in unstimulated TSAd expressing control cells was set to 1.0. Data are means ± SD from three independent experiments. P-values estimated by a 2-tailed paired t-test. c Relative amounts of Vav1 precipitated with anti-Nck antibody as in (b). ns: not statistically significant