Fig. 5
From: Accumulation of the PX domain mutant Frank-ter Haar syndrome protein Tks4 in aggresomes
Aggresomes formed by Tks4R43W mutant colocalizes with the centrosome. COS7 cells were transiently transfected with hCent2-pEGFP-C1 and V5-Tks4 (5a, b, c) or hCent2-pEGFP-C1 and V5-Tks4R43W (5d, e, f) constructs. After 18 h, cells were fixed and stained for Tks4 with anti-V5 antibody (red). Cell nuclei were visualized by DAPI staining. The arrow indicates the localization of centrin2 present at the juxtanuclear region. The scale bar represents 10 μm