Figure 4

Palmitoylated claudin7 promotes cell motility (a) Cells were seeded in FN-coated 24-well plates. Subconfluent cultures were scratched with a pipette tip. Wound healing was followed by light microscopy. Examples and mean ± SD (triplicates) of wound closure are shown (scale bar: 250 μm). (b) Cells suspended in RPMI / 1%BSA were seeded in the upper chamber of a Boyden chamber. The lower chamber contained RPMI/20%FCS. After 16 h of incubation, cells at the lower membrane site were stained with crystal violet and OD 595 nm was determined after lysis; mean ± SD of triplicates. (c) Cells were seeded on BSA-coated 24-well plates. Cell migration was followed for 12 h by videomicroscopy taking pictures every 20 min; examples and the mean ± SD migration of 20 individual cells. (a-c) Significant differences between HEK, HEK-EpC, HEK-EpC-cld7, HEK-EpC-cld7, HEK-EpC^mAG-cld7 and HEK-EpC-cld7^mPalm: *. Palmitoylated cld7 significantly strengthens HEK cell migration.