Figure 2

Comparison of the sensitizing effect of HHT or CHX on TRAIL-induced necroptosis vs. apoptosis in tumor cells. Cells were pretreated with 50 μM zVAD-fmk with or without HHT/CHX (Mz-ChA-1: HHT-0.1 μM, CHX-7.12 μM; HT-29: HHT-1 μM, CHX-17.79 μM; A818-4: HHT-0.1 μM, CHX-35.59 μM; Pt45P1: HHT-0.01 μM, CHX-0.36 μM) and after one hour, 100 ng/ml of TRAIL were added. After 24 h, loss of membrane integrity was measured as a marker for cell death by flow cytometric detection of PI-positive cells. Spontaneous cell death in untreated cells is shown for comparison. Each bar represent the means of two independent experiments with three parallel determinations each, error bars indicate the corresponding SDs. Asterisks indicate statistical significance (t-test; *, P < 0.05; **, P < 0.001; n.s., not significant). Co: Control, N: Necroptosis, A: Apoptosis.