Figure 2

Dynamin-dependent and dynamin-independent effects of dynasore. (A) Dynasore inhibits the GTPase of dynamin, which prevents clathrin-coated endocytosis, including internalisation of LDL receptors in the plasma membrane and inhibits the vesicular H⁺-ATPase, which is involved in cholesterol recycling from endosomes back to the plasma membrane. (B) Dynasore also inhibits the movement of LDL-derived cholesterol from the endolysosomal network to the endoplasmic reticulum. (C) In addition, dynasore impacts cholesterol homeostasis in a dynamin-dependent manner, decreasing the amount of cholesterol in the Golgi apparatus. (D) Dynasore, perhaps by remodelling actin filaments, as well as reducing plasma membrane cholesterol, disperses the organization of lipids in lipid rafts.