EtOH and INK128 induce autophagy. (A) SUDHL-2 cells were treated with 10 μM Chloroquine and with indicated doses of EtOH, INK128 or rapamycin for 6 h. LC3A positive autophagic puncta (arrows show examples) were visualized under fluorescence microscopy. Pictures were taken using a Nikon TE2000S (B) Cells were treated as in (A) with or without Chloroquine. Cell lysates were then analyzed for abundance of LC3 forms by western blotting. β-Actin was used as a loading control. The data are representative of three independent experiments.