Figure 4
From: The Rho GTPase RhoB regulates cadherin expression and epithelial cell-cell interaction
E-cadherin accumulates in endosomes after RhoB knockdown. (A) DU145 cells transfected with the indicated siControl or RhoB siRNAs were stained for F-actin and nuclei (DAPI in merge images) and with antibodies to E-cadherin and EEA1, an early endosome marker. Detail panel shows a magnified image from the boxed region in the merge image. Bars 20 μm. (B) DU145 cells transfected with the indicated siControl or RhoB siRNAs were stained for F-actin and nuclei (DAPI in merge) and with antibodies to E-cadherin and the lysosomal marker LAMP1. Detail panels show magnified images from the boxed regions in the merge images. Bars 20 μm.