Figure 2
From: The Rho GTPase RhoB regulates cadherin expression and epithelial cell-cell interaction
RhoB regulates E-cadherin levels and cell-cell junction integrity. (A) Western blot of lysates from DU145 cells transfected with the indicated siRNAs and probed with the indicated antibodies. Relative levels of E-cadherin expression normalized to α-tubulin loading control are shown below the blot. (B) Quantification of E-cadherin expression from western blots. Graph shows mean expression of E-cadherin +/− s.e.m. relative to siControl-1 from 4 different experiments; *p < 0.05. a.u. arbitrary units. (C) Results from RT-PCR showing mean relative mRNA expression (+/− s.d.; n = 3). **p < 0.005. n.s., not significant. (D) Immunofluorescence showing RhoB and E-cadherin staining in DU145 cells. Images on right show magnified images of the box marked in the merge panels. Bars 50 μm. (E) DU145 cells transfected with the indicated siRNAs were stained for F-actin and nuclei (DAPI in merge images) and with antibodies to E-cadherin and β-catenin. Detail panels show magnified images from the boxed regions in the β-catenin images. Bars 20 μm.