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Table 1 qRT-PCR primer pair sequences for selected genes

From: Murine precision-cut liver slices (PCLS): a new tool for studying tumor microenvironments and cell signaling ex vivo

Gene

Accession number

Forward primer

Reverse primer

Alb

NM_009654.3

CAAGAGTGAGATCGCCCATCG

TTACTTCCTGCACTAATTTGGCA

Cxcl12

NM_013655.4

TGCATCAGTGACGGTAAACCA

TTCTTCAGCCGTGCAACAATC

Gapdh

NM_008084.2

AGGTCGGTGTGAACGGATTTG

TGTAGACCATGTAGTTGAGGTCA

GusB

NM_010368.1

CCGACCTCTCGAACAACCG

GCTTCCCGTTCATACCACACC

Mmp1a

NM_032006.3

CCTTGATGAGACGTGGACCAA

ATGTGGTGTTGTTGCACCTGT

Pdgfa

NM_008808.3

CGATGAGGACCTGGGCTT

TTCTCGGGCACATGGTTAATG

Tgfb2

NM_009367.3

AGAATCGTCCGCTTTGATGTC

TCTGGTTTTCACAACCTTGCT

Tgfb3

NM_009368.3

CCTATCAGGTCCTGGCACTTT

CTGCCCGGAACAGATTGGT

THOC5

NM_172438.3

CTGTGTGCACTTCATGACTCTAAAGA

GAACTCCAGACATTTGGTGATCTCCT

Wnt11

NM_009519.2

GGTGGTACACCGGCCTATG

TCACTGCCGTTGGAAGTCTTG

  1. Alb: albumin (131 nt); Cxcl12: chemokine (C-X-C motif) ligand 12 (146 nt); Gapdh: glyceraldehyde-3 phosphate dehydrogenase (123 nt); GusB: glucuronidase, beta (169 nt); Mmp1a: matrix metallopeptidase 1a (238 nt); Pdgfa: platelet derived growth factor, alpha (232 nt); Tgfb2: transforming growth factor, beta 2 (198 nt); Tgfb3: transforming growth factor, beta 3 (252 nt); THOC5: THO complex subunit 5 homolog (178 nt), Wnt11: wingless-related MMTV integration site 11 (183 nt); (): product size.
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Cell Communication and Signaling

ISSN: 1478-811X