Figure 3
Enforced ILK expression facilitates cell growth by inducing ERK1/2/NF-κB activation. Protein lysates extracted from AGS cells treated with T315 (A) or transfected with Myc-DDK-tagged mutant ILKA262V(B) were immunoprecipitated (IP) with antibodies against ILK. Immunoblots (IB) show the expression of ILK, IQGAP1, and Ras. Plasmids based on pcDNA3.1-Flag-ILK containing full-length ILK (ILK1-452), fragment 171-452 (ILK171-452), fragment 1-170 (ILK1-170) (C), or Myc-DDK-tagged mutant ILKA262V were transfected into MKN45 cells. (D) Coimmunoprecipitation of Flag with the indicated proteins is shown by using western blotting. Compared with pcDNA3.1-Flag (Flag) only, western blots show the expression of phosphorylated ERK1/2 at Tyr202/Thr204 (pERK1/2) (E, F). β-actin was used as an internal control. NF-κB activation (G) and cell growth (H) were also detected in the absence or presence of PD98059 and CAPE. Data are mean ± SD from three independent experiments. **P <0.01 compared with control. ##P <0.01 compared with ILK1-452. Upright triangle, increased expression.