Analysis of gain in isolated retinal tissue. (A) An example WT retina in the isolated tissue ERG preparation. Stimulus range was 4-1350 R*/flash. (B) An example tg retina recorded in the isolated tissue ERG preparation. Stimulus range was 18-980 R*/flash. (C) Response amplitudes for flashes eliciting 260 R*/flash in both WT (black) and tg (red). Responses were normalized to the Rmax for each individual retina to illustrate the difference in the rising phase of the rod photoresponse; WT (black, n = 21) and tg (red, n = 14).