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Figure 1 | Cell Communication and Signaling

Figure 1

From: Activation of C6 glioblastoma cell ceruloplasmin expression by neighboring human brain endothelia-derived interleukins in an in vitro blood-brain barrier model system

Figure 1

hBMVEC up-regulate C6 glioma sCp gene expression. (A) Illustration of C6 glioma cells seeded alone (C6 only) or distal to hBMVEC (EC/-/C6) in transwell. (B) RT-PCR of total C6 glioma RNA examining the presence of sCp and GPI-Cp transcript. (C-E) qPCR of isoform non-specific Cp (C), sCp (D), or GPI-Cp (E) transcript from total RNA collected from C6 glioma cells grown alone (C6) or distal to hBMVEC (EC/-/C6). (F) Serum-free basal media was collected from hBMVEC grown alone in transwell (ECM) and was applied to C6 glioma cells for 24 h. Data represents relative gene expression of sCp in C6 glioma cells obtained by qPCR in control or with ECM addition. A series of paired t-tests were used to determine the significance of the data. *P < 0.05, **P < 0.01, ***P < 0.001. Data are represented as means ± s.d. (n = 3, technical replicates).

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Cell Communication and Signaling

ISSN: 1478-811X