Figure 5

Mast cell-derived exosomes can activate the KIT-SCF signaling pathway in A549 cells. A549 cells treated with exosomes from control cells (HEK 293) or HMC-1 cells were analyzed using Western blot. Phosphorylated and total PI3K, AKT, GSK3β and cyclinD1 were measured by Western blot and to normalize protein loading, samples were also probed for GAPDH. (A). Relative intensity was calculated as follows for p-PI3K (B), p-AKT (C) and p-GSK3β (D); (phosphorylated protein/GAPDH)/(total protein/GAPDH), and for cyclin D1 (E); cyclin D1/GAPDH. All the above data are representative of three independent experiments (n =3). *P <0.05 and **P <0.01.