Figure 3

Mast cell-derived exosomes induce proliferation and migration in A549 cells. (A) BrdU cell proliferation assays were used to detect proliferating A549 cells after co-culturing for 4 hours with HMC-1 exosomes or control exosomes (derived from NIH 3T3 cells). *P <0.05. (B) A549 cells were added to the lower chamber of a Boyden chamber (32 500 cells/well). To the upper chamber 30 μl of the different doses of HMC-1 exosomes were added. Media was used as a control. After 12 hours the number of cells migrated to the lower chamber of the 8 μm pore-sized membrane were analyzed by taking photos and counting the number of cells per visual field. Kruskal-Wallis test followed by Dunn’s multiple comparisons test were used to determine significant differences where all concentrations were only compared to the control. Spearman’s rank correlation coefficient was 0.91 (p <0.0001). p-values; * <0.05, *** <0.001.