Figure 4

Core model simulation of autophagic flux dynamics. The core model was simulated for 100 runs under indicated conditions for 3 hours. For each agent the plotted shaded area corresponds to the 25 and 75 quantile of data. A Time course of vesicle count under FM conditions. B Time course of the vesicle size under FM conditions. C Time course of vesicle count under FM conditions with the addition of BAF. D Time course of the vesicle size under FM conditions with the addition of BAF. E-F Comparison of simulation results to experimentally determined values. E (Right side) Simulated mean vesicle count at the 3 hour time point, with and without the addition of BAF. (Left side) Experimentally measured results (from Figure 3). F (Right side) Simulated mean vesicle size at the 3 hour time point, with and without the addition of BAF. (Left side) Experimentally measured results (from Figure 3). The blue shaded box indicates conditions in the presence of BAF.