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Figure 3 | Cell Communication and Signaling

Figure 3

From: NR5A1 prevents centriole splitting by inhibiting centrosomal DNA-PK activation and β-catenin accumulation

Figure 3

SF-1 depletion induces centriole splitting during G1 phase. (A) Immuno-staining of shluc or shsf1#3 (sh#3) lentivirus infected Y1 cells with antibodies against γ-tubulin (γ-tub) and deploymerized acetylated α-tubulin (Ace-tub). Scale bars are 5 μm. (B and C) Quantification of cells with centriole distance larger than 2 μm in shsf1#3 infected Y1 (B) and MA-10 (C) cells. (D) Vanillin inhibits centriole splitting induced by SF-1 depletion. Quantification of centriole splitting in shluc and shsf1#3 infected Y1 cells in the presence or absence of DNA-PK inhibitor, vanillin. These results are mean ± S.D. from three independent experiments; more than three hundred cells were measured in each individual group.

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Cell Communication and Signaling

ISSN: 1478-811X