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Table 2 Strains and plasmids used in this study

From: The Brucella TIR domain containing proteins BtpA and BtpB have a structural WxxxE motif important for protection against microtubule depolymerisation

Strain or plasmid Relevant characteristics Source
E. coli DH5α DH5α F − 80lacZΔM15 Δ(lacZYA-argF)U169 endA1 recA1 hsdR17 (rK − mK+) supE44 thi-1ΔgyrA96 relA1  
pEGFP-C3 plasmid containing an enhanced GFP expression unit under control of the CMV promoter, Kmr Clontech
pIN271 pEGFP derived plasmid containing GFP-btpA expression unit, Kmr This Study
pIN275 pIN271-derivative carrying btpA W213A This Study
pIN269 pIN271-derivative carrying btpA W213S This Study
pIN274 pIN271-derivative carrying btpA W213F This Study
pIN270 pIN271-derivative carrying btpA E217A This Study
pIN277 pIN271-derivative carrying btpA E217D This Study
pIN262 pIN271-derivative carrying btpA I262S This Study
pIN283 pIN271-derivative carrying btpA G183A This Study
pIN292 pIN271-derivative carrying btpB This Study
pIN293 pIN271-derivative carrying btpB W263S This Study
pIN294 pIN271-derivative carrying btpB E267A This Study