Table 2 Strains and plasmids used in this study
Strain or plasmid | Relevant characteristics | Source |
|---|---|---|
E. coli DH5α | DH5α F − 80lacZΔM15 Δ(lacZYA-argF)U169 endA1 recA1 hsdR17 (rK − mK+) supE44 thi-1ΔgyrA96 relA1 | |
pEGFP-C3 | plasmid containing an enhanced GFP expression unit under control of the CMV promoter, Kmr | Clontech |
pIN271 | pEGFP derived plasmid containing GFP-btpA expression unit, Kmr | This Study |
pIN275 | pIN271-derivative carrying btpA W213A | This Study |
pIN269 | pIN271-derivative carrying btpA W213S | This Study |
pIN274 | pIN271-derivative carrying btpA W213F | This Study |
pIN270 | pIN271-derivative carrying btpA E217A | This Study |
pIN277 | pIN271-derivative carrying btpA E217D | This Study |
pIN262 | pIN271-derivative carrying btpA I262S | This Study |
pIN283 | pIN271-derivative carrying btpA G183A | This Study |
pIN292 | pIN271-derivative carrying btpB | This Study |
pIN293 | pIN271-derivative carrying btpB W263S | This Study |
pIN294 | pIN271-derivative carrying btpB E267A | This Study |