Figure 3

Blocking of AhR ligand binding does not increase CXCL8 and CCL5 responses in BEAS- 2B cells. Cells were incubated with 0.5 μM of the AhR antagonist α-naphtoflavone (ANF) for 30 min prior to exposure for 18 h to 20 μM B[a]P, 1-NP, 1-AP or vehicle (DMSO) alone. CYP1A1 (A) levels were measured by real-time PCR, while CXCL8 (B) and CCL5 (C) levels in the medium were measured by ELISA as described under “Materials and methods”. The results are expressed as mean ± SEM (A: n = 1 (triplicate determinations); B/C: n = 5). *Significantly different from unexposed controls; †Significant effect of ANF.