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Figure 1 | Cell Communication and Signaling

Figure 1

From: AhR and Arnt differentially regulate NF-κB signaling and chemokine responses in human bronchial epithelial cells

Figure 1

P65 is required for CXCL8 but not CCL5 responses in 1- NP- or 1- AP- exposed BEAS- 2B cells. Cells were transfected with siRNA against p65 (siP65) or non-targeting control siRNA (siNT), and exposed to 20 μM 1-NP, 1-AP or vehicle (DMSO) alone. CXCL8 (A) and CCL5 (B) gene expression were measured after 6 h by real-time PCR. CXCL8 (C) and CCL5 (D) protein levels in the medium were measured by ELISA after 18 h exposure as described under “Materials and methods”. Efficiency of transfection was assessed by Western blotting (E). The results are expressed as mean ± SEM (A/B: n = 1 (triplicate determinations); C/D: n ≥ 3; E: representative blot, n = 3). *Significantly different from unexposed controls; †Significantly different from cells transfected with non-targeting siRNA.

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Cell Communication and Signaling

ISSN: 1478-811X